PLEASE DON'T SUBMIT ANY JOB YET. STILL EDITING
If encounter problems, please contact Daisuke Kihara (dkihara@purdue.edu) or Xiao Wang (xiaowang20140001@gmail.com) or Genki Terashi (gterashi@purdue.edu)
Please simply click "Upload" when you filled all input fields.
If you are not sure, just use threshold 0, our model can automatically detect the structure regions.
Please adjust the contour level for the best fit. Set a contour level that is sufficiently low to cover the entire protein region as a continuous volume. DMcloud does not check map regions below (lower than) the contour level you specified. On the other hand, if you specify the contour level too low, it will take longer time for the program to check every voxels above the contour level.
After adjusting the contour level on the sliding window click "Update Contour Value" to visualize the volume. Note that the map may not be visualized when the map is large and the specified contour level is too low or the specified contour level is too high and the browser runs out of memory.
AlphaFold2 modeled structure in pdb format. Please combine all single-chain structures in one PDB file, separated by "TER" for different chains' records. The chain ID does not matter, for identical chains, you only need to provide the chain records once in the pdb format. Although submitting AF2 model is optional we highly recommend to provide it because the accuracy can often largely improve. To obtain AF2 models or to use Alphafold3, see the tutorial.
Please simply ignore this field if you do not plan to use AlphaFold2 structure.
Please select Yes if you have any two or more chains are identical. That is important of correct chain assignment for identical chains.
