ComplexModeler combines DiffModeler(seq) and CryoREAD to automatically build full protein-DNA/RNA complex structures from cryo-EM maps at up to 10 Å resolution (5–10 Å may work but DNA/RNA regions will not be as accurate).

If you encounter problems, please contact Daisuke Kihara (dkihara@purdue.edu) or Han Zhu (zhu773@purdue.edu).

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Example Input

Map File: 20031.mrc
Sequence: 20031.fasta
Contour level: 0.03
Resolution: 4.3

Example Result

View example job

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Please click "Upload" when you have filled in all input fields.

cryo_em_map :

Determine an appropriate contour level for fitting your protein structure. You can also find it interactively on the submission page after uploading your map.

The tool will not work properly without an appropriate contour level.

contour_level :

The map resolution is required as input. If the resolution is 2 Å or better (high resolution), the diffusion step in DiffModeler will be skipped.

resolution :

Paste sequences directly into the text fields on the submission page. Protein sequence is required (can be partial); DNA/RNA sequence is optional. You can also upload a FASTA file (see the tutorial for the format).

fasta :

Job Note (Optional):
This note will help you distinguish jobs later.