No instructions have been provided for this algorithm yet.
MAINMAST is a de novo modeling protocol to build an entire protein 3D model directly from near-atomic resolution EM map.
MainMast only works for single-chain, for multi-chain, please use DeepMainMast and DiffModeler(seq).
If encounter problems, please contact Daisuke Kihara (dkihara@purdue.edu), Genki Terashi(gterashi@purdue.edu).
Please make sure your contour level is lower than your focused region. This is absolute density threshold, not standard deviation.
Contour level: 1.0
Result Example:Result ExampleTutorial
Reference: https://www.nature.com/articles/s41467-018-04053-7
Please adjust the contour level for the best fit. Set a contour level that is sufficiently low to cover the entire protein region as a continuous volume. mainmast does not check map regions below (lower than) the contour level you specified. On the other hand, if you specify the contour level too low, it will take longer time for the program to check every voxels above the contour level.
After adjusting the contour level on the sliding window click "Update Contour Value" to visualize the volume. Note that the map may not be visualized when the map is large and the specified contour level is too low or the specified contour level is too high and the browser runs out of memory.
