MAINMAST is a de novo modeling protocol to build an entire protein 3D model directly from near-atomic resolution EM map.

MainMast only works for single-chain, for multi-chain, please use DeepMainMast and DiffModeler(seq).

If encounter problems, please contact Daisuke Kihara (, Genki Terashi(

Example one chain map of EMD-6374
Input Map file:6374.mrc

Please make sure your contour level is lower than your focused region. This is absolute density threshold, not standard deviation.

Contour level: 1.0

Result Example:Result Example



Please simply click "Schedule Job" when you filled all input fields.