MAINMAST is a de novo modeling protocol to build an entire protein 3D model directly from near-atomic resolution EM map.
MainMast only works for single-chain, for multi-chain, please use DeepMainMast and DiffModeler(seq).
If encounter problems, please contact Daisuke Kihara (dkihara@purdue.edu), Genki Terashi(gterashi@purdue.edu).
Example one chain map of EMD-6374 Input Map file:6374.mrc
Please make sure your contour level is lower than your focused region. This is absolute density threshold, not standard deviation.
Contour level: 1.0
Result Example:Result Example
Tutorial
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Reference: https://www.nature.com/articles/s41467-018-04053-7
Please simply click "Schedule Job" when you filled all input fields.